Khoom
![2- [TRIS (Hydroxymethyl) methancino]-1-eethanesulfonic acid; cas No. 7365-44-8 Featured Duab](https://cdn.globalso.com/pengnuochemical/2-Trishydroxymethylmethylamino-1-ethanesulfonic-acid-7365-44-8.png)
2- [TRIS (Hydroxymethyl) Methylramino]-1-Ethanesulfonic acid; CAS No.: 7365-44-8
![2- [TRIS (Hydroxymethyl) methancino]-ethanesulfonic acid 7365-44-8](http://www.pengnuochemical.com/uploads/2-Trishydroxymethylmethylamino-1-ethanesulfonic-acid-7365-44-8.png)
Txhais lus hais tias: Chebi: 39036; 2 - {[hydroxy-1,1-bis (hydrothanesulfonate; n-tris (hydroxymethyl) methyl-2-ammonioethanesulfonate
Cov cuab yeej tshuaj ntawm 2- [TRIS (Hydroxymethyl) methancino]-ethanesulfonic acid
Winds Ntag / Xim: Dawb Flash Crystalline Hmoov
● Melting point: ~ 223-225 ° C
● Cov ntsiab lus tsis zoo: 1.57
● PKA: 7.5 (ntawm 25 ℃)
● Psa: 135.47000
● Ceev: 1.554 g / cm3
● LogP: -14880
● Temp cia.: Khaws ntawm RT.
● Solubility.:h2o: 1 m ntawm 20 ° C, ntshiab, tsis muaj kob
● Dej solubility .: soluble
● XlogP3: -5.8
Itydrogen BRonor Pub pub Suav: 4
Hydrogen BRRong Txais Txais Suav: 6
Resot Rotatable Bond COUNTER: 6
● Muaj tseeb tiag tshua: 229.06200837
● Hnyav Hnyav Atome ath
Inclexity: 220
Cov Ntaub Ntawv Safty
● POTOSSGRAM (S):
Xi
● Cov cai phom sij: xi
● Cov nqe lus: 36/37/38
● Cov nqe lus kev nyab xeeb: 22-24 / 25-36-26
Zoo siv
● Canonical smiles:C (CS (= O) (= O) [O -] [NH2 +] C (CO) (CO) CO
● Siv:Ib tug qauv analog mus rau tis tsis. Lamological tsis. Tes yog ib qho feem ntau siv buffering tus neeg sawv cev.
Cov lus qhia ntxaws ntxaws
2- [TRIS (Hydroxymethyl) methancino]-ethanesulfonic acid, tseem hu ua cov tes lossis n-2-hydroxyethylpiperazine-n'-ethanesulfonic acid, yog ib qho kev tshawb fawb biochemical feem ntau siv hauv kev tshawb fawb biochemical thiab molecular biology biology.
Kua roojFeem ntau ua haujlwm raws li kev sim hauv kev kuaj sim vim nws muaj peev xwm tswj tau cov kab ntawv ntev ruaj khov, tshwj xeeb hauv thaj tsam 6.5 txog 8.5. Nws kuj tseem muaj cov roj teeb roj ntau qis, uas ua rau nws siv rau kev siv hluav taws xob spectrophottometric no, tso cai rau cov kev sim txhim kho.
Kua roojFeem ntau siv hauv kev siv roj ntsha thiab biochemical siv, cov protein purification, electrophoresis, thiab cell kev cai xov xwm tawm.
Nws yog tsim nyog sau tseg tias cov tes yog hygroscopic, lub ntsiab lus nws tau yooj yim nqus dej los ntawm ib puag ncig, yog li nws yog qhov tseem ceeb rau kev khaws cia thiab ua kom zoo los tswj nws cov hauj lwm zoo.
Daim ntawv thov
2- [TRIS (Hydroxymethyl)-ethadlamino]-1-et) yog qhov tseem ceeb compound feem ntau paub txog nws cov khoom tsis zoo. Qee qhov ntawm nws cov ntsiab lus siv yog:
Tus neeg sawv cev buffering:Tes yog feem ntau siv raws li kev sib xyaw ua ke ntawm biochemical thiab kev sim biology molecular. Nws pab txhawm rau tswj cov qauv qib ruaj khov, tshwj xeeb tshaj yog nyob rau hauv thaj tsam ntawm 6.5 txog 8.5
Roj ntsha thiab biochemical cia:Tes yog siv nyob rau hauv ntau biological thiab biochemical cia siab uas tswj xyuas qhov tseeb pH rau kev ua tau zoo. Nws pab txhawm rau tswj cov ph ntawm cov tshuaj assay thiab muab kev ruaj khov rau cov tshuaj tiv thaiv.
Cov tshuaj muaj protein:Tes yog feem ntau siv nyob rau hauv cov txheej txheem protein purification vim nws qhov muaj peev xwm disffering thiab kev sib xyaw nrog enzyme cov haujlwm. Nws pab tswj kom muaj kev ruaj khov thiab kev ua si ntawm cov protein thaum lub sij hawm ua kom muaj cov kauj ruam.
Electrophoreses:Tes ua hauj lwm ua tus buffering Cheebtsam hauv gel electrophoresis cov txuj ci, tshwj xeeb hauv polyacrylamide gel electrophoresis (nplooj). Nws muab cov pal ruaj khov rau kev sib cais protein thiab tsiv teb tsaws.
Cell Kev Cai Xov Xwm:Tes yog suav nrog hauv cell kev cai xov xwm kom tswj tau cov pH tas mus li rau kev ua kom zoo tshaj plaws ntawm tes ntawm tes thiab muaj kev loj hlob. Nws pab tswj cov ph ntawm cov kab lis kev cai nruab nrab thiab muab ib puag ncig zoo rau lub hlwb.
Nws yog qhov tseem ceeb kom nco ntsoov tias cov ntaub ntawv tshwj xeeb thiab concentration ntawm cov tes yuav sib txawv raws li kev sim cov kev sim. Kev ua kom zoo, cia, thiab mloog kom paub meej hauv cov khoom lag luam yog qhov tseem ceeb kom ntseeg tau tias nws qhov ua tau zoo thiab kev nyab xeeb.
