Khoom
Hepes; Cas.:7365-45-9
Txhais lus hais tias: 1- [4- (2-Hydrothley) -1-Piperazinyl] Equanesulfonic Acid; 4- Hydroxyulfonic Acid; 4- Hydroxyethen) Piperazine-1- (2-Ethanesulfonic acid); n- (hydroxyethyl) piperazine-n'-hydrotulfonic acid; nsol 166663; n '- hydroxyethyl) piperazine-n- ethanesulfonic acid); n'-2-hydroxyethylpiperazine-n-2-hydrozine-piperazine-ethanesulfonicacid); 4- hydrothancidfonicacid, 4- hydroxyethulfonicacid) -;
Tshuaj Khoom Ntawm Hepes
Winds Ntag / Xim: Dawb Flash Crystalline Hmoov
Vapor siab: 0pa ntawm 25 ℃
● Melting point: 234-238 ° C
● Cov ntsiab lus tsis zoo: N20 / D 1.339
● Bowing taw tes: 408 ℃ [101 325 PA]
● PKA: 7.5 (ntawm 25 ℃)
● Psa:89.46000
Wensity: 1.325 g / cm3
● LogP: -0.55930
● Cov temp.:2-882882
● Solubility.:h2o: 1 m ntawm 20 ° C, ntshiab, tsis muaj kob
● Dej Solubility.:Soluble
● XlogP3: -4.1
Hydrogen BRonor Pub pub Suav: 2
Hydrogen BRRongle Txais Txais Suav: 5
Resot Rotatable Bond cee Suav: 4
● KEEB MEEM KEEB: 238.09872823
● Hnyav Hom Dav: 15
Inclexity: 254
Cov Ntaub Ntawv Safty
● POTOSSGRAM (S):
Xi
● Cov cai phom sij: xi
● Cov nqe lus: 36/37/38
● Cov nqe lus nyab xeeb: 24 / 25-22-36-26
Zoo siv
Canonical luag nyav:C1cn (CC [NH +] 1CCS (= O) (= O) [O -]) CCO
Nqe lus piav qhia:Hepes tau piav qhia tias yog ib qho zoo tshaj plaws txhua lub hom phiaj ua rau muaj rau kev tshawb fawb roj ntsha. Ntawm Biological Ph, lub molecule yog zwitterionic, thiab yog qhov ua tau zoo li tsis muaj buffer ntawm pH 6.8 txog 8.2 (PKA 7.55). Nws yog feem ntau yog siv nyob rau hauv cell kev coj noj coj ua ntawm kev sib txuam ntawm 5mm txog 30 hli. HEPES tau siv nyob rau hauv ntau cov ntawv thov, suav nrog cov nqaij cov kab lis kev cai. Nws yog ib qho uas siv tau los ua cov kev cai ntawm tes cell kev cai xov hauv huab cua. Hepes pom nws cov kev siv hauv cov kev sim vitro ntawm mg.
Siv:Tsis muaj tshuaj lom rau ntawm tes. Nws yog siv los ua hydrogen ion tsis tswj, uas tuaj yeem tswj hwm qhov ntau ntu ntev rau lub sijhawm ntev. Qhov kev xav tau yog 10-50mmol / l. Feem ntau, 20mmol / lhepes nyob rau hauv cov kev daws kev noj haus huv tuaj yeem tau txais kev muaj peev xwm tsis muaj peev xwm. Lamological tsis. HEPES yog ib qho tsis muaj buffer rau roj ntsha sciency, tshwj xeeb siv nyob rau hauv cell kab lis kev cai kom tswj cov physiological Ph. Nws ua ib qho kev ua haujlwm ntawm kev sib xyaw, uas yog siv rau hauv kev npaj ntawm buffers. Nws tau piav raws li ib qho zoo tshaj plaws txhua lub hom phiaj ua rau siv hauv kev ntsuas ntsev, dulbeco tus qauv ntsuas ntsev, uas yog siv rau kev coj ua ntawm cov kab lis kev cai ntawm kev coj noj coj ua ntawm kev kuaj xyuas lub cav.
Cov lus qhia ntxaws ntxaws
HepesSawv ntsug rau 2- (4- (2-hydroxyethyl) piperazin-1-yl) ethanesulfonic acid. Nws yog tus neeg sawv cev buffering feem ntau siv hauv kev tshawb fawb roj ntsha thiab biochemical. Cov hepes pab tswj kav hauv xov tooj ntawm tes xov xwm, raws li nws muaj peev xwm rau ob leeg lees txais thiab pub cov protulating, yog li tswj cov hom. Nws cov khoom siv tshuaj lom thiab cov khoom ua rau nws ua tau zoo nyob rau hauv tas mus li thiab zoo pH rau ntau cov roj ntsha roj ntsha thiab enzymatic. Cov hepes yog feem ntau siv nyob rau hauv cell kab lis kev cai, protein purification, thiab molecular biology cov tswv yim.
Daim ntawv thov
HEPES muaj ob peb daim ntawv thov hauv ntau cov kev tshawb fawb. Qee cov kev siv ntawm cov hepes yog:
Cellure Culture: Cov hepes yog feem ntau siv raws li ib qho kev sib xyaw ua ke hauv cell kev cai xov xwm kom tswj tau ntev ntev pH ib puag ncig. Nws pab tswj cov kev hloov thib v los ntawm cov haujlwm metabolic tau tshwm sim los ntawm cov haujlwm metabolic thiab cov khoom pov thawj ntawm cov khoom pov tseg hauv cov xov xwm ntawm tes.
Enzyme nco:Cov hepes feem ntau siv raws li tus neeg sawv cev buffering nyob rau hauv enzyme assectays tswj hwm pH tas li rau kev ua haujlwm zoo ntawm cov enzymes. Nws pab kom qhov tshwm sim raug.
Electrophoreses:Cov hepes yog siv los ua tus neeg sawv cev buffering nyob rau hauv electrophoresis cov txuj ci zoo li polyacrylamide gel electrophoresis (nplooj) thiab agarose gel electrophoresis. Nws pab tswj cov phev xav tau rau kev sib cais zoo tshaj plaws rau kev sib cais zoo nkauj thiab tsom xam ntawm Macromolecules xws li cov protein thiab nucleic acids.
Cov tshuaj muaj protein:Hepes qee zaum suav nrog cov tsis muaj protein purement buffificate kom tswj tau lub sijhawm xav txog lub sijhawm ua ntu zus ntawm cov txheej txheem lim tshuaj. Nws pab tswj kom muaj kev ruaj khov thiab kev ua si ntawm cov protein thaum lub sijhawm ua kom muaj kev lim.
Molecular cov txheej txheem biology:Hepes tuaj yeem siv ntau hauv ntau cov txheej txheem xyoob ntoo, xws li PV (polymerase Nws pab ua kom ua kom ua kom zoo tshaj plaws ntawm enzymes thiab ua kom ntseeg tau tias yog thiab ntseeg tau.
Nws yog ib qho tseem ceeb kom nco ntsoov tias qhov kev sib txawv ntawm cov hepes thiab nws daim ntawv thov yuav sib txawv raws li kev sim thiab cov cai.
