SynonymsHEPES sodium ntsev; 75277-39-3; HEPES hemisodium ntsev; 103404-87-1; Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl) ethanesulfonate; sodium 2-[4-(2-hydroxyethyl) Piperazin-1-yl] ethanesulfonate; HEPES (sodium); C8H17N2NaO4S; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium ntsev; 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium ntsev; 4-(2-Hydroxyineethyl-1-piper) ethanesulfonic acid sodium ntsev; Sodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate; UNII-Z9FTO91O8A; Z9FTO91O8A; sodium; 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate; DTXSID40444EI47; CHEBI; 278-169-7; 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium ntsev (1:1); N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, sodium ntsev; HEPES sodium ntsev, > = 99.5% (titration); sodium Hepes; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium ntsev; MFCD00036463; HEPES, sodium ntsev; HEPES sodium ntsev, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-HPSNa ntsev; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid) hemisodiumsalt; HEPES sodium ntsev, biochemical Qib;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0692086;FT-0692086 8 610086 N'-hydroxyethyl-N-piperazineethanesulfonate; HEPES sodium ntsev, BioXtra, > = 99.0% (titration); HEPES sodium ntsev, Vetec (TM) reagent qib, 96%; W-104397; Q27120698; Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl) ethanesulfonate; 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium ntsev; 4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium ntsev; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt; N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium ntsev; HEPES sodium ntsev, BioPerformance Certified, haum rau cell kab lis kev cai, > = 99.5%
● Cov tsos / Xim: hmoov dawb
● Vapor Siab: 0Pa ntawm 25 ℃
● Melting Point: 234 ° C
● PKA: 7.5 (ntawm 25 ℃)
● PSA:92.2900 Nws
● Ceev: 1.504 [ntawm 20 ℃]
● LogP:-0.90190
● Cia Temp.: Khaws ntawm RT.
● Sensitive.: Hygroscopic
● Solubility.:H2O: 1 M ntawm 20 ° C, ntshiab, tsis muaj xim
● Dej Solubility.: Nws yog soluble hauv dej.
● Hydrogen Bond Donor Count: 1
● Hydrogen Bond Txais Tus Nqi: 6
● Rotatable Bond suav: 5
● Exact Mass: 260.08067248
● Heavy Atom suav: 16
● Kev nyuaj: 272
● Daim duab:
● Hazard Codes:
● Cov Lus Qhia Txog Kev Nyab Xeeb: 22-24/25
Chav Kawm Tshuaj:Lwm Cov Kev Siv -> Cov Khoom Siv Hluav Taws Xob
Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Kev siv:HEPES sodium ntsev tov yog tsim rau kev tshawb fawb kab lis kev cai ntawm tes. Nws tuaj yeem siv rau hauv kev npaj cov tshuaj tsis haum ntawm HEPES. Nws tuaj yeem siv los ua tsis tau thaum lub sijhawm phosphorylation kev soj ntsuam hauv permeabilized fibroblasts. Nws tuaj yeem siv los tsim cov tshuaj lim dej tsis zoo, siv los txiav txim siab dawb thyroxin hauv cov ntshav los ntawm radioimmunoassay. HEPES sodium ntsev tov yog tsim rau siv nyob rau hauv kev tshawb fawb kab lis kev cai ntawm tes. Nws tuaj yeem siv rau hauv kev npaj cov tshuaj tsis haum ntawm HEPES. Nws tuaj yeem siv los ua tsis tau thaum lub sij hawm phosphorylation kev soj ntsuam hauv permeabilized fibroblasts. Nws tuaj yeem siv los tsim cov tshuaj lim dej tsis zoo, siv los txiav txim siab dawb thyroxin hauv cov ntshav los ntawm radioimmunoassay.
HEPES sodium ntsev, tseem hu ua 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium ntsev, yog ib hom kev siv HEPES. Nws yog zwitterionic organic compound uas ua raws li tus neeg sawv cev buffering hauv kev tshawb fawb lom neeg thiab biochemical.
HEPES sodium ntsev feem ntau yog siv hauv xov tooj ntawm tes kab lis kev cai xov xwm thiab tshuaj lom neeg tsis zoo vim nws muaj peev xwm tswj tau qhov pH ruaj khov nyob ib ncig ntawm lub cev (pH 7.2 - 7.6). Nws yog soluble hauv dej thiab muaj cov tshuaj toxicity tsawg, ua rau nws haum rau ntau yam kev siv.
Cov sodium ntsev daim ntawv ntawm HEPES yog nyiam nyob rau hauv ntau daim ntawv thov raws li nws txhim khu solubility thiab stability, piv rau dawb acid daim ntawv. Nws yog cov khoom lag luam muaj nyob hauv cov hmoov dawb crystalline uas tuaj yeem yaj tau yooj yim hauv dej los npaj cov kev daws teeb meem.
Cov kws tshawb fawb feem ntau siv HEPES sodium ntsev hauv cov txheej txheem biology molecular, kab lis kev cai ntawm tes, kev soj ntsuam enzyme, protein purification, thiab lwm yam kev sim biochemical uas tswj pH tseem ceeb heev. Nws muaj peev xwm buffering thiab compatibility nrog cov kab mob lom neeg ua rau nws yog ib qho kev xaiv nrov rau kev tswj xyuas cov xwm txheej zoo rau kev ua haujlwm thiab kev ruaj ntseg ntawm ntau yam kab mob lom.
Ua ntej siv HEPES sodium ntsev nyob rau hauv ib daim ntawv thov tshwj xeeb, nws yog ib qho tseem ceeb uas yuav tsum tau tshuaj xyuas cov ntaub ntawv, sab laj nrog cov neeg muag khoom cov lus pom zoo, thiab ua kom zoo dua qhov concentration thiab pH ntau yam raws li qhov kev sim tshwj xeeb.
HEPES sodium ntsev pom muaj ntau daim ntawv thov nyob rau hauv ntau yam kev tshawb fawb lom thiab biochemical. Qee qhov kev siv tseem ceeb suav nrog:
Cell Culture:HEPES sodium ntsev feem ntau ntxiv rau hauv xov tooj ntawm tes kab lis kev cai xov xwm kom muaj pH ruaj khov nyob rau hauv lub cev muaj zog thiab muab qhov chaw tsim nyog rau cov hlwb kom loj hlob thiab loj hlob.
Buffering Agent:HEPES sodium ntsev feem ntau ua haujlwm ua tus neeg sawv cev hauv cov tshuaj lom neeg lom neeg thiab cov kev daws teeb meem, suav nrog kev tshuaj xyuas enzyme, kev ua kom huv ntawm cov protein, thiab cov txheej txheem molecular biology. Nws pab tswj lub pH tas li los ntawm kev tiv thaiv kev hloov hauv acidity lossis alkalinity.
Electrophoresis: HEPES sodium ntsev yog siv los ua ib qho tsis nyob hauv gel electrophoresis kom muaj pH ruaj khov thaum lub sij hawm sib cais ntawm nucleic acids los yog proteins. Nws tiv thaiv kev hloov pauv hauv pH uas tuaj yeem cuam tshuam rau kev tsiv teb tsaws thiab kev sib cais ntawm cov molecules hauv cov gel.
Protein Stability: HEPES sodium ntsev yog ntxiv rau cov kev daws teeb meem protein kom ruaj khov lawv cov qauv thiab tswj lawv cov dej num. Nws pab tswj qhov zoo pH ib puag ncig tsim nyog rau cov protein ruaj khov thiab ua haujlwm.
Enzyme Kev Ua Haujlwm: HEPES sodium ntsev yog siv los ua ib qho tsis nyob hauv cov tshuaj tiv thaiv enzymatic los tswj cov pH uas xav tau rau kev ua haujlwm zoo enzyme. Nws pab xyuas kom meej tias cov enzymes ua haujlwm ntawm lawv qhov ua tau zoo tshaj plaws thiab raug.
Live Cell Imaging:HEPES sodium ntsev feem ntau suav nrog hauv cov duab thaij duab rau kev sim duab nyob hauv cell. Nws lub peev xwm buffering pab tswj qhov xav tau pH thiab tiv thaiv kev hloov pauv uas yuav cuam tshuam rau kev muaj peev xwm thiab fluorescence ntawm cov hlwb.
Molecular Biology Techniques: HEPES sodium ntsev yog dav siv nyob rau hauv ntau yam molecular biology hom kev kawm, xws li DNA los yog RNA cais, PCR, DNA sequencing, thiab protein tsom xam. Nws lub peev xwm buffering tso cai rau qhov tseeb thiab txhim khu kev qha thaum cov txheej txheem no.
Nws yog ib qho tseem ceeb uas yuav tsum nco ntsoov tias daim ntawv thov tshwj xeeb thiab cov ntsiab lus ntawm HEPES sodium ntsev tuaj yeem sib txawv nyob ntawm qhov kev sim thiab cov txheej txheem lom neeg hauv kev kawm. Yog li ntawd, nws raug nquahu kom sab laj cov ntaub ntawv thiab cov lus pom zoo rau kev siv.