Khoom
Heepes ntsev ntsev; CAS No.: 75277-39-3
Txhais lus hais tias: Hee sodium ntsev; 75277-39-3; (sodium); c8h17n2N2N2N2S; 4- Hydroadfonic acid, 4- ethanesulfonic acid, 4- ethandium ntsev; 1-ethanesulium piperelylonic acid sev ntsev; sodium 4- (2-hydroxyethyl) piperazin-1-YLETTA91OA; DTXSAUG; 278-169-7; 1-PiperazineEtulfonic acid, 4- (2-hydroxyethulfonic acid, sodium ntsev, sodium ntsev, uepes sodium ntsev, uces ntsev; mob siab; n- (2-hydroxyethyl) piperazine-n '- (2-etchesulfonic acid) sodium ntsev; heepes ntsev; heepes sodium ntsev, 98%; c8h18n2o4s.na; yog-ithtwevxfv-hhffl3184; (1m); tox21_302155; HB5187; N- Fydroxyethylonicacid) HemesodfiumSalt; heepes sodium ntsev; heepes sodium ntsev; heepes sodium ntsev; heepes sodium ntsev; Qib; AKOS015897769; AKOS015925964204; NCGC00255277; FT-0610868; A838366; sodium N'-hydroxyethyl-n-piperazinehanesulfonate; heepes sodium ntsev,> = lub hnub nyoog); hepes sodium ntsev, vetec (tm) 96%; W-104397; sodium220698; sodium2- (2- hydrothanate; 4- hydroxyethulfonate; 4- hydrothanate; 4- hydroxyethulfonate; Monosodium ntsev; n- (2-hydroxy-piperazine-n-ethanesulfonic acid) sodiumsuldium ntsev; tsim nyog rau cell kev cai,> = 99.5%
Cov Khoom Muaj Tshuaj Ntawm Kev Ua Si Sodium Ntsev
Winds Ntag khaub Race / Xim: Cov hmoov dawb
Vapor siab: 0pa ntawm 25 ℃
● Melting point: 234 ° C
● PKA: 7.5 (ntawm 25 ℃)
● Psa:92.29000
● Ceev: 1.504 [ntawm 20 ℃]
● LogP: -0.90190
● Cia Champ. :Ttore ntawm RT.
● Rhiab .:hygroscopic
● Solubility.:h2o: 1 m ntawm 20 ° C, ntshiab, tsis muaj kob
● Dej solubility.:it yog soluble hauv dej.
Hydrogen BRONG pub pub nyiaj Suav: 1
Hydrogen BRRong Txais Txais Suav: 6
Resetable Nyiaj Txiag Khov Khoom Noj: 5
● Muaj tseeb tiag tshua muaj: 260.08067248
● Hnov hnyav atom: 16
● Cov lus teb nyuaj: 272
Cov Ntaub Ntawv Safty
● POTOSSGRAM (S):
● Cov kab mob txaus ntshai:
Proferements Kev Nyab Xeeb Kev Nyab Xeeb: 22-24 / 25
Zoo siv
Tshuaj lom neeg:Lwm Yam Siv -> Kev Raug Mob Buffers
Canonical luag nyav:C1cn (CCN1CO) CCS (= O) (= O) [O -]. [NA +]
Siv:HEPES SERPACE NTSE NTSWG Cov ntsev Kev daws yog tsim rau kev tshawb fawb ntawm tes kab lis kev cai. Nws yuav raug siv rau hauv kev npaj ntawm kev daws teeb meem ntawm kev mob siab. Tej zaum nws yuav raug siv los ua kev ua yeeb yam thaum lub sijhawm phosphorylation cia nyob rau hauv permeumilized fibroblasts. Tej zaum nws yuav raug siv rau compose ib tug nyuaj lim ntshav tsis, siv hauv kev txiav txim siab ntawm cov dawb thyroxin hauv cov ntshav dej ntawm radioimmunoassay. HEPES SERPES SUV DEJ DEJ yog qhov tsim rau kev siv hauv kev tshawb fawb ntawm tes kab lis kev cai. Nws yuav raug siv rau hauv kev npaj ntawm kev daws teeb meem ntawm kev mob siab. Tej zaum nws yuav raug siv los ua kev ua yeeb yam thaum lub sijhawm phosphorholdation nyob rau hauv permeumilized fibroblasts. Tej zaum nws yuav raug siv rau compose ib tug nyuaj lim ntshav tsis, siv hauv kev txiav txim siab ntawm cov dawb thyroxin hauv cov ntshav dej ntawm radioimmunoassay.
Cov lus qhia ntxaws ntxaws
Heepes ntsev ntsev, tseem hu ua 4- (2-hydroxyethyl) piperazine-1-eethanesulfonic acid sev ntsev, yog ib hom kev nyiam ua. Nws yog cov organic zwitteric organic uas ua raws li tus neeg sawv cev buffering hauv kev tshawb fawb roj ntsha thiab biochemical.
Hepes ntsev ntsev feem ntau yog siv nyob rau hauv cell kev cai xov xwm tawm vim nws lub peev xwm los tswj cov qib ruaj nyob ib ncig ntawm lub dag zog (pH 7.2 - 7.6). Nws yog soluble heev hauv dej thiab muaj cov tshuaj lom tsawg, ua rau nws haum rau ntau yam kev siv.
Cov kua txiv ntsev ntsev yog nyiam nyob rau hauv ntau daim ntawv thov raws li nws tau txhim kho solubility thiab ruaj khov, piv rau cov tshuaj laus. Nws yog kev lag luam muaj xws li cov hmoov dawb crystalline uas tuaj yeem yooj yim yaj hauv dej los npaj cov kev daws teeb meem ua haujlwm.
Cov kws tshawb nrhiav feem ntau siv hepes sodium ntsev hauv cov tswv yim biology, enzyme kev ua yeeb yam, thiab lwm yam kev sim protein, thiab lwm yam kev sim proteinical uas yog qhov tseem ceeb heev. Nws cov khoom muaj peev xwm ua kom haum thiab sib xyaw nrog kev siv roj ntsha ua rau nws xaiv tau zoo rau kev ua ub no thiab kev sib luag ntawm ntau yam lwg roj ntsha molecules.
Ua ntej siv cov tshuaj pleev ua kom zoo nkauj hauv cov ntaub ntawv tshwj xeeb, sab laj kom saib cov ntaub ntawv, thiab ua kom pom cov lus pom zoo thiab ua kom muaj cov kev xav tau tshwj xeeb.
Daim ntawv thov
HEPES SODINE SETFACT SEFT pom kev ua haujlwm thoob plaws hauv ntau txoj haujlwm tshawb fawb roj ntsha thiab cov chaw tshawb fawb biochemical. Qee qhov nws tseem ceeb siv suav nrog:
Cellure Culture:Hepes ntsev ntsev feem ntau tau ntxiv rau cov kab kev cai ntawm tes kom tswj tau lub cuab yeej ruaj khov thiab muab ib puag ncig zoo rau cov hlwb kom loj hlob thiab proliferate.
Tus neeg sawv cev buffering:HEPES SERPACY NTSE YUAV TSUM UA HAUJ LWM RAU HAUV LUKMERING NYOB RAU HAUV LUZICATING NYOB RAU HAUV LWJ XEEM, suav nrog enzyme presays, thiab molecular biology. Nws pab tswj cov pH tsis tu ncua los ntawm kev tawm tsam kev hloov pauv hauv acidity lossis alkalinity.
Electrophoreses: HEPES SODEY NTSE NYEM yog siv los ua cov ntas nyob rau hauv gel electrophoresis kom tswj tau cov kua ruaj khov ntawm nucleic acids lossis cov protein. Nws tiv thaiv kev hloov pauv hauv PHO uas tuaj yeem cuam tshuam kev tsiv teb tsaws thiab sib cais qauv ntawm cov lwg me hauv cov gel.
Protein stability: Hepes ntsev ntsev tau ntxiv rau cov kev daws teeb meem protein kom ruaj khov lawv cov qauv thiab tswj lawv cov haujlwm. Nws pab tswj cov kab ntawv sau qib siab xav tau rau cov protein ruaj khov thiab ua haujlwm.
Enzyme kev ua: HEPES SERPACE NTSE NYEM yog siv los ua ib qho ntas hauv enzymatic cov tshuaj yuav tsum tau tswj cov yam ntxwv xav tau. Nws pab xyuas kom meej tias cov enzymes muaj nuj nqi ntawm lawv cov kev ua tau zoo tshaj plaws thiab raug.
Nyob ntawm tes ntawm tes:Hepes ntsev ntsev yog feem ntau suav nrog hauv cov xov xwm kos rau cov kev sim ntawm tes nyob hauv lub cev. Nws cov peev txheej tsis muaj zog pab tswj kav phev uas xav tau?
Molecular cov txheej txheem biology: HEPES SERPACE NTSE NYEEM UA HAUJ LWM HAUV ntau cov tswv yim biology, suav nrog DNA lossis RNA Kev rho tawm, PCR, DNA tsom xam. Nws buffering muaj peev xwm tso cai rau cov txiaj ntsig raug thiab txhim khu kev qha thaum cov txheej txheem no.
Nws yog ib qho tseem ceeb kom nco ntsoov tias cov ntaub ntawv tshwj xeeb thiab concentration ntawm kev ua siab ntev ntsev ntsev yuav sib txawv raws li kev sim thiab kev txheeb xyuas kev kawm. Yog li ntawd, nws raug nquahu los sab laj rau cov ntaub ntawv thiab tus neeg xa khoom pom zoo rau kev siv kev zoo siab.
