Khoom
Hepps; CAS No.: 16052-06-5
Txhais lus hais tias: HEPPS; 16052-06-5; epps; 3- hydroxyRropanesulfonic acid; Acid; 1-piperazyprropanesulfonic acid, 4- (2-hydrothethyl) piperazin-1-hydrotelethyl acid); 4- (2-hydroxy-piperazine-1-hydrozypropanesulphonic acid; einecs 240-198-8; BN 3957385; 8jn5pqd99 4C; 4- Hydroxyethylane Sulphineprore acid; MFCD00006160; 3- Hydroxyethyl) -0-Piperazinyl] -1-Propanesulfonic acid; ep1; n-2-hydroxyethylpiperazine-n'-3-propanesuldfonic acid; schemb9c; Sepsid306003; Epps,> = 99.5% (tunration); HY-D0873; C9-H20-N2-O426002; epps, neps0% (t); vetec (tm) Qib, 99%; AC-24630; AS-69273; EPPS, Bioxtra,> = 99.5% (Kaumil); LS-113260; CS-113260; FT-0616538; H01848; H01848; acid; n-2-hydroxyylpiperazine-n-3-propanesulfonic acid; Q15635; (2-hydroxy) acid; 4- (2-hydroxyethyl) piperazine-1-ulanesulphonic acid); 3- fydrotelethyl) piperazinyl) propanesulforonic acid (HEPPS); 3- 4- Hydroxyethylas-eps, 99.5% (10- Hydroxyethyl) Piperazin-1-YL] Propylsulfonyloxonium
Tshuaj Khoom Ntawm Hepps
Winds Ntag / Xim: Dawb Flash Crystalline Hmoov
● Melting point: 237-239 ° C (Lit.)
● Cov ntsiab lus tsis zoo: 1.537
● PKA: 8.0 (ntawm 25 ℃)
● Psa:89.46000
Wensity: 1.285 G / cm3
● LOGP: -0.16920
● Cia Champ. :Ttore ntawm RT.
● Solubility.:h2o: 1 m ntawm 20 ° C, ntshiab, tsis muaj kob
● Dej solubility.: ansoluble hauv dej. 25.2 g / l (20 ° C)
● XlogP3: -3.6
Hydrogen BRonor Pub pub Suav: 2
Hydrogen BRRong Txais Txais Suav: 6
Resot Rotatable Bond COUNTER: 6
● Muaj tseeb muaj tseeb: 252.11437830
● Hnov hnyav atom: 16
● Cov neeg nyuaj: 280
Cov Ntaub Ntawv Safty
● POTOSSGRAM (S):
Xi
● Cov cai phom sij: xi
● Cov nqe lus: 36/37 / 38-22-22-22
● Cov nqe lus muaj kev nyab xeeb: 24 / 25-36-26
Zoo siv
Tshuaj lom neeg:Nitrogen Teb -> Piperazines
Canonical luag nyav:C1cn (CCN1CCCS (= O) (= O) O) CCO
Siv:Epps yog ib txwm siv zoo? S tsis. Nws yog feem ntau siv ua separator hauv Ultratin Isoelectric tsom iav thiab txhim kho kev daws teeb meem ntawm phosphogluucuase. Nws yog siv los ua tus neeg sawv cev buffering hauv biology thiab biochemistry.
Cov lus qhia ntxaws ntxaws
EPPS sawv rau n- (2-hydroxyethyl) piperazine-n '- (3-propanesulfonic acid). Nws yog cov neeg sawv cev zwitteric tsis zoo uas yog feem ntau siv hauv kev tshawb fawb roj ntsha thiab biochemical kev tshawb fawb, yog qee cov ntsiab lus tseem ceeb hais txog EPPS:
Cov Khoom Siv Buffing:Epps yog ib tus neeg sawv cev ua haujlwm zoo uas pab tswj cov pH tas li hauv kev sim ua ntu zus. Nws muaj tus nqi PKA ntawm 8.09, uas ua rau nws haum rau buffering nyob rau hauv lub ph ntau ntawm 7.0-9.0.
Stability:EPPS cov ntawv qhia tau zoo tshaj qhov kub thiab txias dua li qhov kub thiab txias hloov pauv piv rau lwm cov neeg sawv cev thiab phosphate buffers. Qhov kev ruaj khov no ua rau nws haum rau kev siv hauv cov kev sim bichchemical thiab kev sim tshiab uas yuav tsum tau tswj kav.
Kev Siv Hluav Taws Xob:Epps yog feem ntau siv nyob rau hauv ntau cov kev tshawb fawb roj ntsha, xws li cov protein purification, enzyme cia, thiab DNA / RNA Kev rho tawm. Nws cov teeb meem muaj peev xwm thiab kev ruaj khov ntawm lub phob pheeb suab physiological PHEEJ Nws muaj nuj nqis hauv cov ntawv thov no.
Cellure Culture:EPPS kuj tseem siv tau los ua tus neeg sawv cev buffering hauv cell kev cai xov xwm. Nws pab tswj cov chaw ruaj khov loj kom txaus rau kev loj hlob thiab saib xyuas ntawm qee yam xov tooj ntawm tes.
Ions thiab sib xyaw:Epps yog ib qho zwitterionic compound, txhais tau nws muaj ob qho tib si tsub thiab tsis zoo. Cov yeeb yaj kiab no tso cai rau nws kom ua tau zoo tsis muaj ob qho tib si acidic thiab cov kev daws teeb meem yooj yim. Nws yog sib xws nrog ntau yam lwg me me thiab yog paub tias muaj kev cuam tshuam tsawg tsawg nrog kev cuam tshuam enzymatic.
Thaum siv epps, nws yog ib qho tseem ceeb kom ua raws li cov lus qhia kev pom zoo thiab PH cov lus qhia muab los ntawm cov khw. Ib qho ntxiv, ib txwm ceev faj thiab ua raws cov kev nyab xeeb kev nyab xeeb thaum tuav ib qho tshuaj lom neeg lossis tus neeg sawv cev buffering.
Daim ntawv thov
Hepps (n- (2-hydroxyethyl) piperazine-n'-3-propanesulfonic acid) yog ib qho uas muaj kev tshawb fawb roj ntsha thiab biochemical. Qee qhov ntawm nws cov ntawv thov suav nrog:
Cellure Culture: Hepps yog siv los ua tus neeg sawv cev buffering nyob rau hauv cell kev cai xov xwm pab kom ib puag ncig tas li thiab muab cov kev loj hlob ntawm tes thiab muaj kev cuam tshuam. Nws pab ruaj khov rau cov pH nyob hauv kev siv dag zog ntawm lub cev thiab tsis txhob muaj kev cuam tshuam zoo ntawm cov txheej txheem cellular.
Enzyme nco:Hepps feem ntau yog siv los ua cov tsis sib haum xeeb hauv enzyme cia siab rau kev ua kom zoo rau hom PH uas yuav tsum muaj rau cov haujlwm siab tshaj plaws. Nws cov khoom lag luam tsis muaj peev xwm pab tswj cov pH ruaj khov, ua kom muaj tseeb thiab txhim khu kev qha enzyme ntsuas.
Cov tshuaj muaj protein:Hepps tuaj yeem siv tau raws li kev ua tsis tau hauv cov txheej txheem protein purification. Nws pab tswj cov chaw ruaj khov thiab kev ua si ntawm cov protein thaum lub sij hawm ua kom muaj cov kauj ruam, xws li kem chromatography los yog protein purification siv affination cim npe.
Electrophoreses:Hepps qee zaum tau siv los ua tus tsis tau nyob hauv gel electrophoresis rau kev sib cais thiab tsom xam ntawm cov protein thiab nucleic acids. Nws lub peev xwm p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p p P
Molecular cov txheej txheem biology:Hepps tuaj yeem siv los ua cov txheej txheem hauv ntau hom molecular cov tswv yim, suav nrog PCR (polymerase cov tshuaj tiv thaiv), DNA kev sib cav, thiab kev sib txheeb. Nws lub peev xwm buffaber ensures muaj kev coj zoo rau kev ua tiav thiab ua tau zoo.
Cov kev tshawb fawb uas muaj cov tshuaj tiv thaiv cov tshuaj tiv thaiv kabxeeb:Hepps yog zoo rau kev kawm cov tshuaj pleev kua mis protein vim nws qhov qis dua cov khoom siv. Nws yog feem ntau siv los ua cov tsis tau hauv cov kev sim uas muaj kev cuam tshuam nrog kev ua kom muaj nuj nqi lossis kev sib raug cai ntawm cov tshuaj tua tsiaj.
Nws yog ib qho tseem ceeb kom nco ntsoov tias qhov kev pom zoo tshaj plaws thiab Ph ntawm Hepps tuaj yeem sib txawv raws li cov ntawv thov tshwj xeeb lossis kev sim. Yog li ntawd, nws raug nquahu los sab laj rau cov ntaub ntawv lossis cov chaw muag khoom pom zoo rau kev siv tsim nyog thiab kev xav txog HEPPS hauv koj txoj kev sim.
