Khoom
Cov kav dej; CAS No.: 5625-37-6
Cov lus txhais lus:1,4-Piperazinediethane sulfonic acid;
1,4-piperazinediethanesulphonic acid;
1,4-piperazinehanesulfonate; piperazine-n, n'-bis (2-ethanesulfonic acid);
Piperazine-N, N'-BIS (2-Ethanesulfonic acid), sodium ntsev; kav dej
Cov Khoom Muaj Tshuaj Ntawm Kav Dej
Winds Ntag / Xim: Dawb / Vinin Crystalline Hmoov
● Melting point:> 300 ° C (Lit.)
● Cov ntsiab lus tsis zoo: 1.6300 (kwv yees)
● PKA: 6.8 (ntawm 25 ℃)
● Psa: 131.98000
Wensity: 1.505 G / cm3
● LogP: 0.41700
● Cia Champ. :Ttore ntawm RT.
● Solubility.:0.1 M Naoh: 0.25 g / ml, ntshiab, tsis muaj kob
● Dej solubility.: ansoluble hauv dej.
● XlogP3: -6.7
Hydrogen BRonor Pub pub Suav: 2
● Hydrogen BRRongle Txais Txais Suav: 8
Resot Rotatable Bond COUNTER: 6
● Muaj pes tsawg cov huab hwm: 302.06062865
● Hnov Cov Atom Nruab Nrab: 18
● Nyuaj: 397
Purity / zoo
99% * Cov ntaub ntawv los ntawm cov neeg xa tawm
1,4-PiperazineBis (Ethanesulfonicacid) 99% * cov ntaub ntawv los ntawm cov khoom siv reagent
Cov Ntaub Ntawv Safty
● POTOSSGRAM (S):
Xi
● Cov cai phom sij: xi
● Cov nqe lus: 36/37/38
● Cov nqe lus kev nyab xeeb: 22-24 / 25-37 / 39-26
Msds Cov Ntaub Ntawv
Zoo siv
Tshuaj lom neeg:Lwm Yam Siv -> Kev Raug Mob Buffers
Canonical luag nyav:C1cn (CCN1CCS (= O) (= O) O) CCS (= O) (= O) O
Siv:Piperazine-N, N'-Bis- (2-Eethanesulphonic acid) ultra -pure> 99.5%) yog ib qho tseem ceeb ntawm kev siv tshuaj lom neeg. 1,4-piperazinediethatulfonic acid, piperazine-n, n'-ete tsim nyog siv ib qho ntawm pk2 ntawm cov kav dej yog 6.8 ntawm 20 degrees Celsius. Tus neeg sawv cev buffering nrog PKA ze ntawm lub cev Ph.Pipes nyob ze ntawm lub ph.perazine-n, n'-eethanesulfonic acid)] yog nquag siv los ua tus neeg sawv cev buffering hauv biochemistry. Nws yog ib qho ethanesulfonic acid tsis tsim los ntawm zoo li al. Hauv 1960s. Cov kav dej muaj PKA nyob ze ntawm lub physiological PH uas ua rau nws muaj txiaj ntsig hauv kev ua haujlwm ntawm tes kab lis kev cai. Nws tau raug sau cia kom txo cov ntaub ntawv lipid thaum ua kom muaj glutaraldeDEYDE iDDDitional.additional.com cov kav dej, sesquisodium ntsev; Kav Kav Dej Dipotassium; Kav kav disodium; Kav dej, sodium ntsev. Buffers tuaj yeem npaj los ntawm kev daws teeb meem ntawm paus rau cov kav dej dawb acid, lossis los ntawm kev sib xyaw ua ke ntawm cov roj ntsha Monosodium thiab cov ntsev sib luag thiab cov lus tsis sib xws Cov kav dej, yog siv buffering tus neeg sawv cev hauv Biochemistry. Cov kav dej muaj PKA (6.76 ntawm 25 ° C) nyob ze ntawm lub physiological ph uas ua rau nws muaj txiaj ntsig hauv kev ua kab lis kev cai. Cov kev cai tau tshaj tawm txog kev siv cov kav dej rau kev sib cais ntawm glyoxylated RNA hauv agarose gels, Nuclease S1 daim phiaj ntawm RNAs, thiab hauv Ribonucion kev tiv thaiv ascocols. Cov kav dej tau siv los ua cov tsis tau nyob hauv GlutaraldeHyde txhim kho ntawm cov protein crystallization. Cov kav dej tau pom zoo siv rau hauv Buffers rau hauv cov kav hlau vitro yog qhov kev qhia ntawm cov khoom siv uas tsis yog cov tshuaj tiv thaiv cov protein li protein. Cov kav dej tau siv hauv kev coj noj coj ua hauv xov tooj rau cov kev siv ntawm cov engineering ntawm cov thermostable mutein hauv Escherichia Coli.
Cov lus qhia ntxaws ntxaws
Cov kav dej dawb acid, tseem hu ua 1,4-piperazinesulfonic acid, yog ib tug zoo tsis ceeb rau muaj pka tus nqi zoo ib yam li lub cev zoo tib yam physiological ph. Xws li, kav dej dawb acid yog siv los ua cov tsis tau nyob hauv biochemical kev tshawb fawb.Pipes yog qhov muaj txiaj ntsig zoo rau cov qib 6.1 - 7.5. Cov kav dej tsis muaj peev xwm los tsim ib qho tseem ceeb nrog cov hlau ions thiab raug pom zoo rau kev siv cov tsis sib xyaw nrog cov hlau ions. Cov kav dej muaj ntau yam ntawm cov ntawv thov thiab feem ntau siv nyob rau hauv candgine crystallization, raws li ib qho chaw nyob hauv isoelricography. Qhov tsis zoo no muaj peev xwm tsim cov radicals thiab yog vim li no tsis haum rau redox cov kev tawm tsam. Nws yog qhov tsim nyog rau kev siv nyob rau hauv bicinchinic acid (BCA) assay. Solubility ntawm cov kav dej nce ntxiv thaum cov kua qaub dawb yog hloov mus rau cov roj ntsha ua kua rau cov kav dej txhaws rau cov kua qaub thiab cov ph
Cov kav dej yog ib tus tswv cuab ntawm ethanesulfonic series, ua ntej tshaj tawm los ntawm kev hloov pauv ntawm txhua lwm cov kuab tshuaj, qhov tsawg kawg nkaus nqus dej hauv txhua yam Txij li thaum nws PMA ntawm 37 ° C yog nyob ze lub phobiological Ph, cov kav dej muaj cov ntawv thov hauv kev ua kab lis kev cai.
Cov lus qhia ntxaws ntxaws
Cov kav dej, tseem hu ua Piperazine-N, N'-BIS (2-Ethanesulfonic acid), yog cov tsis siv tshuaj lom neeg thiab cov kev tshawb fawb biochemical. Nws yog lub zwitterionic sib xyaw uas pab tswj hwm pH nyob rau hauv cov kev daws teeb meem aqueous.
Ib qho ntawm thawj cov ntawv thov ntawm cov kav dej yog nyob hauv thaj teb molecular biology thiab biochemistry, qhov chaw uas siv raws li kev npaj thiab khaws cov tshuaj lom neeg. Cov kav dej muaj tus nqi PKA muaj kwv yees li ntawm 7.46, uas ua rau nws zoo tagnrho rau kev tswj hwm qib phot 6.7 txog 37 degrees Celsius).
Cov kav dej yog qhov tshwj xeeb tshwj xeeb rau kev tswj cov pH ruaj khov thaum lub sijhawm ua haujlwm xws li protein purification, enzyme cia, thiab gel electrophoresis. Nws pab kom txo qhov thib ph thiaj li yuav cuam tshuam rau cov qauv thiab kev ua haujlwm ntawm cov protein thiab cov enzymes.
Lwm daim ntawv thov ntawm cov kav dej yog hauv kev tshawb fawb ntawm tes kab lis kev cai. Nws yog feem ntau siv raws li kev ua tsis tau rau cov xov xwm ntawm tes tawm. Cov kav dej pab tswj kom muaj qhov tsis tu ncua pH hauv qhov nruab nrab ntawm qhov nruab nrab, uas yog qhov tseem ceeb rau kev loj hlob thiab kev sib raug ntawm cov hlwb. Nws tseem muab ib puag ncig ruaj khov rau kev kawm cov txheej txheem cellular thiab ua kev sim.
Cov kav dej tseem tab tom siv hauv ntau cov txuj ci kev ntsuas, xws li kua chromatography thiab capillary electrophoresis. Nws cov teeb meem fuffering thiab pH ruaj khov ua rau nws haum rau kev siv hauv cov txuj ci no, qhov twg precise py kev tswj yog qhov tseem ceeb rau cov txiaj ntsig raug.
Tsis tas li ntawd, cov kav dej tau pom cov ntawv thov hauv cov yeeb tshuaj yeeb tshuaj thiab cov khoom xa tuaj. Nws tuaj yeem siv los ua kev ua yeeb yam hauv cov tshuaj ua kom zoo rau cov tshuaj ruaj khov thiab txhim kho cov tshuaj haum.
Zuag qhia tag nrho, cov kav dej yog cov neeg sawv cev rau ntau yam nrog cov kev siv hauv roj ntsha thiab biochemical, kab lis kev cai ntawm tes, thiab tshuaj yeeb yam. Nws lub peev xwm los tswj cov qib ruaj khov ua rau nws muaj txiaj ntsig zoo hauv ntau daim teb science.
